Unfortunately, i did not get any problem with the burning software.
I have used a DX48BT2 (latest bios) with a compatible CPU & memory on the tested list.
Optical Drive SATA: Lite ON
Windows Vista 32 bit
Nero Software to test and Power Iso as well
Hard drive connected to first two sata ports
Optical drive connected to last sata ports.
Bios Settings SATA configured as IDE/RAID
Try to test your optical drive
Try to test your system with another Optical Drive.
Other than this, i dont have any other suggestions.