Hi Tony, not an uncommon situation for the SSD to be no longer seen by the toolbox. I had the same problem with a 330/120G and was able to recover it and make it work, however, the method erased all the data so you may want to look at other options first. IF there are any and I don't hold much hope for that.
Windows File Explorer could see the drive so I went into
Selected the errant 330SSD and formatted it.
Rebooted and ran the Intel toolbox again and it once again found the SSD.
A word of caution if you do get the SSD back, messing with the alignment is not an option in the Toolbox, so it should have given you an indication that it is probably not a good thing to do. If Intel thinks it is something that needs tweaking then they would have included it in the Toolbox. Stick to Trim only.
I’m sorry for the trouble you have been experiencing. I noticed this thread has been an open issue for a while and would like to suggest you contact Intel Customer Support for any additional troubleshooting and/or warranty support that still may be required.