Not exactly - The midplane is designed around a SAS expander. It has one SFF-8087 connector to SAS RAID adapter, providing SAS connectivity between the SAS RAID card and 12 SAS/SATA drives.
However, the RS2BL040 RAID card has only one SFF-8087 connector, meaning you have no port left for internal drives.
In your scenario, you'll need a RAID card with at least two SFF-8087 connectors. And I highly recommend you choose a RAID card from the Tested hardware list. So SRCSASJV seems a good choice for you.
Another option is to connect 12 hotswap drives to the RAID controller, and two internal drives to onboard SATA ports and configure RAID 1 using ESRT2.